Detailed information of TA system TA155818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5195749..5196344
• Replicon: chromosome
• Accession: NZ_CP053028
• Organism: Pseudomonas aeruginosa PAO1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: HKX07_RS24040
• Protein ID: WP_003113526.1
• Coordinates: 5196066..5196344 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HKX07_RS24035
• Protein ID: WP_003113527.1
• Coordinates: 5195749..5196054 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKX07_RS24010	5190861..5191151	-	291	WP_033992920.1	DUF5447 family protein	-
HKX07_RS24015	5191363..5191635	-	273	WP_003115921.1	hypothetical protein	-
HKX07_RS24020	5192165..5193262	+	1098	WP_169917322.1	protein phosphatase 2C domain-containing protein	-
HKX07_RS24025	5193265..5194314	+	1050	WP_003113529.1	serine/threonine protein kinase	-
HKX07_RS24030	5194311..5195384	+	1074	WP_003113528.1	serine/threonine protein kinase	-
HKX07_RS24035	5195749..5196054	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
HKX07_RS24040	5196066..5196344	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKX07_RS24045	5196673..5198901	+	2229	WP_003113525.1	TonB-dependent receptor	-
HKX07_RS24050	5198971..5199618	-	648	WP_003095021.1	carbonate dehydratase	-
HKX07_RS24055	5199680..5200918	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T155818 WP_003113526.1 NZ_CP053028:c5196344-5196066 [Pseudomonas aeruginosa PAO1]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T155818 NZ_CP068816:2577130-2577408 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT155818 WP_003113527.1 NZ_CP053028:c5196054-5195749 [Pseudomonas aeruginosa PAO1]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT155818 NZ_CP068816:2577408-2577692 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: V6ALY3