Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 109329..110056 Replicon plasmid pKPN361-2
Accession NZ_CP053018
Organism Klebsiella pneumoniae strain KPN361

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag HJ591_RS27905 Protein ID WP_004118600.1
Coordinates 109745..110056 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HJ591_RS27900 Protein ID WP_000990392.1
Coordinates 109329..109748 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJ591_RS27870 105016..105378 - 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -
HJ591_RS27875 105426..105779 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HJ591_RS27880 106095..106688 + 594 WP_044348674.1 hypothetical protein -
HJ591_RS27885 107112..107771 + 660 WP_000078540.1 hypothetical protein -
HJ591_RS27890 107768..108097 + 330 WP_004118613.1 hypothetical protein -
HJ591_RS27895 108090..109292 + 1203 WP_003031541.1 hypothetical protein -
HJ591_RS27900 109329..109748 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HJ591_RS27905 109745..110056 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
HJ591_RS27910 110267..110647 + 381 Protein_128 gluconate permease -
HJ591_RS27915 110697..111392 - 696 WP_004118595.1 lactate utilization protein C -
HJ591_RS27920 111385..112812 - 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
HJ591_RS27925 112823..113542 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA1 / qacE / sul1 / mph(A) / aph(3')-Ia / qnrS1 / blaLAP-2 / aac(3)-IId - 1..220791 220791


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T155802 WP_004118600.1 NZ_CP053018:c110056-109745 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T155802 NZ_CP068815:c4207353-4207039 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT155802 WP_000990392.1 NZ_CP053018:c109748-109329 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT155802 NZ_CP068815:c4207589-4207356 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References