Detailed information of TA system TA155798

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37704..37973
• Replicon: plasmid pKPN361-1
• Accession: NZ_CP053017
• Organism: Klebsiella pneumoniae strain KPN361

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HJ591_RS26415
• Protein ID: WP_001312861.1
• Coordinates: 37815..37973 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37704..37769 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJ591_RS26390	33414..33941	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HJ591_RS26395	33999..34232	+	234	WP_000006003.1	DUF905 family protein	-
HJ591_RS26400	34293..36316	+	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
HJ591_RS26405	36385..36819	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HJ591_RS26410	36816..37535	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	37547..37771	+	225	NuclAT_0	-	-
-	37547..37771	+	225	NuclAT_0	-	-
-	37547..37771	+	225	NuclAT_0	-	-
-	37547..37771	+	225	NuclAT_0	-	-
-	37704..37769	+	66	-	-	Antitoxin
HJ591_RS26415	37815..37973	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HJ591_RS26420	38211..38588	-	378	Protein_50	hypothetical protein	-
HJ591_RS26425	38888..39184	+	297	WP_001272251.1	hypothetical protein	-
HJ591_RS26430	39295..40116	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HJ591_RS26435	40413..41060	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HJ591_RS26440	41337..41720	+	384	WP_000124981.1	relaxosome protein TraM	-
HJ591_RS26445	41911..42597	+	687	WP_015059009.1	PAS domain-containing protein	-
HJ591_RS26450	42691..42918	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..169824	169824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T155798 WP_001312861.1 NZ_CP053017:37815-37973 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T155798 NZ_CP068815:c3651923-3651621 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT155798 NZ_CP053017:37704-37769 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin