Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2020386..2020950 Replicon chromosome
Accession NZ_CP052936
Organism Lacticaseibacillus paracasei strain CLP-C10

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag HKJ34_RS09600 Protein ID WP_016363448.1
Coordinates 2020386..2020688 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag HKJ34_RS09605 Protein ID WP_003570051.1
Coordinates 2020681..2020950 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ34_RS09585 (HKJ34_09725) 2015699..2016256 + 558 WP_003584278.1 helix-turn-helix transcriptional regulator -
HKJ34_RS09590 (HKJ34_09730) 2016681..2018858 - 2178 WP_003570057.1 Tex family protein -
HKJ34_RS09595 (HKJ34_09735) 2019094..2019786 - 693 WP_003570055.1 hypothetical protein -
HKJ34_RS09600 (HKJ34_09740) 2020386..2020688 - 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
HKJ34_RS09605 (HKJ34_09745) 2020681..2020950 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HKJ34_RS09610 (HKJ34_09750) 2021324..2021977 - 654 WP_003570049.1 N-acetyltransferase -
HKJ34_RS09615 (HKJ34_09755) 2022022..2022651 - 630 WP_273646514.1 DUF2399 domain-containing protein -
HKJ34_RS09620 (HKJ34_09760) 2022714..2023040 - 327 WP_003584272.1 YrdB family protein -
HKJ34_RS09625 (HKJ34_09765) 2023033..2023437 - 405 WP_003570045.1 MerR family transcriptional regulator -
HKJ34_RS09630 (HKJ34_09770) 2023635..2024861 + 1227 WP_003570043.1 hypothetical protein -
HKJ34_RS09635 (HKJ34_09775) 2025058..2025945 + 888 WP_003570041.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T155729 WP_016363448.1 NZ_CP052936:c2020688-2020386 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T155729 NZ_CP068813:262336-262443 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT155729 WP_003570051.1 NZ_CP052936:c2020950-2020681 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT155729 NZ_CP068813:c262278-262224 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References