Detailed information of TA system TA155721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41527..41791
• Replicon: plasmid pC21-3
• Accession: NZ_CP052880
• Organism: Escherichia coli strain C21

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HJD21_RS23680
• Protein ID: WP_001331364.1
• Coordinates: 41639..41791 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	41527..41584 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJD21_RS23665	37865..38935	-	1071	WP_000151579.1	IncI1-type conjugal transfer protein TrbB	-
HJD21_RS23670	38954..40162	-	1209	WP_169711497.1	IncI1-type conjugal transfer protein TrbA	-
HJD21_RS23675	40380..41366	-	987	WP_001257838.1	hypothetical protein	-
-	41527..41584	-	58	NuclAT_0	-	Antitoxin
-	41527..41584	-	58	NuclAT_0	-	Antitoxin
-	41527..41584	-	58	NuclAT_0	-	Antitoxin
-	41527..41584	-	58	NuclAT_0	-	Antitoxin
HJD21_RS23680	41639..41791	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HJD21_RS23685	41863..42114	-	252	WP_001291964.1	hypothetical protein	-
HJD21_RS23690	42587..43815	+	1229	WP_089541817.1	IS3 family transposase	-
HJD21_RS23695	44109..44285	-	177	WP_001054901.1	hypothetical protein	-
HJD21_RS23700	44617..44826	+	210	WP_000062602.1	HEAT repeat domain-containing protein	-
HJD21_RS23705	44898..45560	-	663	WP_124983586.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..87627	87627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T155721 WP_001331364.1 NZ_CP052880:41639-41791 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T155721 NZ_CP068812:c4301426-4301208 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT155721 NZ_CP052880:c41584-41527 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin