Detailed information of TA system TA155718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4549419..4550021
• Replicon: chromosome
• Accession: NZ_CP052877
• Organism: Escherichia coli strain C21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HJD21_RS21670
• Protein ID: WP_000897305.1
• Coordinates: 4549710..4550021 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HJD21_RS21665
• Protein ID: WP_000356397.1
• Coordinates: 4549419..4549709 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJD21_RS21640	4545364..4546266	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HJD21_RS21645	4546263..4546898	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HJD21_RS21650	4546895..4547824	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HJD21_RS21655	4548154..4548396	-	243	WP_001086388.1	hypothetical protein	-
HJD21_RS21660	4548615..4548833	-	219	WP_001611167.1	ribbon-helix-helix domain-containing protein	-
HJD21_RS21665	4549419..4549709	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HJD21_RS21670	4549710..4550021	-	312	WP_000897305.1	hypothetical protein	Toxin
HJD21_RS21675	4550250..4551158	+	909	WP_001162704.1	alpha/beta hydrolase	-
HJD21_RS21680	4551222..4552163	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HJD21_RS21685	4552208..4552645	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HJD21_RS21690	4552642..4553514	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HJD21_RS21695	4553508..4554107	-	600	WP_001295269.1	glucose-1-phosphatase	-
HJD21_RS21700	4554206..4554991	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T155718 WP_000897305.1 NZ_CP052877:c4550021-4549710 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155718 NZ_CP068812:3739158-3739367 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155718 WP_000356397.1 NZ_CP052877:c4549709-4549419 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155718 NZ_CP068812:3739364-3739666 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7