Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1831411..1831913 Replicon chromosome
Accession NZ_CP052877
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HJD21_RS08795 Protein ID WP_000767829.1
Coordinates 1831659..1831913 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HJD21_RS08790 Protein ID WP_001259255.1
Coordinates 1831411..1831662 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS08765 1826589..1827656 - 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HJD21_RS08770 1827656..1828726 - 1071 WP_169711485.1 histidinol-phosphate transaminase -
HJD21_RS08775 1828729..1830027 - 1299 WP_000009595.1 histidinol dehydrogenase -
HJD21_RS08780 1830033..1830932 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HJD21_RS08785 1831078..1831128 - 51 WP_001364200.1 his operon leader peptide -
HJD21_RS08790 1831411..1831662 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HJD21_RS08795 1831659..1831913 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HJD21_RS08800 1831996..1832820 + 825 WP_000754737.1 SDR family oxidoreductase -
HJD21_RS08805 1832866..1833795 + 930 WP_000803351.1 LysR family transcriptional regulator -
HJD21_RS08810 1834010..1834072 + 63 WP_010723108.1 membrane protein YoeI -
HJD21_RS08815 1834062..1835420 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HJD21_RS08820 1835599..1836657 + 1059 WP_029397759.1 transport protein YeeE -
HJD21_RS08825 1836671..1836898 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T155702 WP_000767829.1 NZ_CP052877:1831659-1831913 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T155702 NZ_CP068812:c1624749-1624438 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT155702 WP_001259255.1 NZ_CP052877:1831411-1831662 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT155702 NZ_CP068812:c1624441-1624022 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References