Detailed information of TA system TA155696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 588539..589338
• Replicon: chromosome
• Accession: NZ_CP052877
• Organism: Escherichia coli strain C21

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: HJD21_RS02850
• Protein ID: WP_000347266.1
• Coordinates: 588539..589003 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HJD21_RS02855
• Protein ID: WP_073461570.1
• Coordinates: 589003..589338 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJD21_RS02820	583540..583974	-	435	WP_032182759.1	PTS sugar transporter subunit IIA	-
HJD21_RS02825	583992..584870	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HJD21_RS02830	584860..585639	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HJD21_RS02835	585650..586123	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HJD21_RS02840	586146..587426	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HJD21_RS02845	587675..588484	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HJD21_RS02850	588539..589003	-	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HJD21_RS02855	589003..589338	-	336	WP_073461570.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HJD21_RS02860	589487..591058	-	1572	WP_001273741.1	galactarate dehydratase	-
HJD21_RS02865	591433..592767	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
HJD21_RS02870	592783..593553	+	771	WP_073461569.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T155696 WP_000347266.1 NZ_CP052877:c589003-588539 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T155696 NZ_CP068812:236822-236998 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12416.98 Da
• Isoelectric Point: 4.6890

>AT155696 WP_073461570.1 NZ_CP052877:c589338-589003 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIDDDE

Nucleotide

• Length: 336 bp

>AT155696 NZ_CP068812:237044-237460 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2