Detailed information of TA system TA155692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 81383..82110
• Replicon: plasmid p3846III
• Accession: NZ_CP052873
• Organism: Enterobacter cloacae strain 3849

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A482LZ08
• Locus tag: HJI43_RS24310
• Protein ID: WP_045898923.1
• Coordinates: 81383..81694 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HJI43_RS24315
• Protein ID: WP_103181184.1
• Coordinates: 81691..82110 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJI43_RS24285	76729..77403	-	675	Protein_84	efflux RND transporter permease subunit	-
HJI43_RS24290	77409..77546	-	138	Protein_85	hypothetical protein	-
HJI43_RS24295	77591..78004	-	414	WP_103181181.1	hypothetical protein	-
HJI43_RS24300	78663..79076	+	414	WP_130588776.1	hypothetical protein	-
HJI43_RS24305	79936..80334	+	399	WP_103181183.1	H-NS histone family protein	-
HJI43_RS24310	81383..81694	+	312	WP_045898923.1	type II toxin-antitoxin system HigB family toxin	Toxin
HJI43_RS24315	81691..82110	+	420	WP_103181184.1	helix-turn-helix domain-containing protein	Antitoxin
HJI43_RS24320	82088..82281	-	194	Protein_91	hypothetical protein	-
HJI43_RS24325	82295..83170	-	876	Protein_92	IS3 family transposase	-
HJI43_RS24330	83242..84410	+	1169	WP_096998908.1	IS3 family transposase	-
HJI43_RS24335	84418..84700	-	283	Protein_94	transposase	-
HJI43_RS24340	85320..85469	-	150	WP_158250578.1	hypothetical protein	-
HJI43_RS24345	86692..86979	-	288	WP_015572079.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190697	190697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12347.07 Da
• Isoelectric Point: 9.7248

>T155692 WP_045898923.1 NZ_CP052873:81383-81694 [Enterobacter cloacae]
MHVISKEPFEEAAKQYPNDSLAVRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T155692 NZ_CP068811:4699551-4699928 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15475.62 Da
• Isoelectric Point: 4.3573

>AT155692 WP_103181184.1 NZ_CP052873:81691-82110 [Enterobacter cloacae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAIELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQYRLSYSDLKEEIGSKSLVSQILSGQRSLTIMHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT155692 NZ_CP068811:4699094-4699462 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A482LZ08