Detailed information of TA system TA155677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 744083..744813
• Replicon: chromosome
• Accession: NZ_CP052870
• Organism: Enterobacter cloacae strain 3849

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: HJI43_RS03610
• Protein ID: WP_022646552.1
• Coordinates: 744083..744397 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HJI43_RS03615
• Protein ID: WP_023305145.1
• Coordinates: 744394..744813 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJI43_RS03585	740164..741249	+	1086	Protein_692	cellulase family glycosylhydrolase	-
HJI43_RS03590	741155..742339	-	1185	WP_023298963.1	multidrug efflux MFS transporter EmrD	-
HJI43_RS03595	742515..742933	-	419	Protein_694	EamA family transporter	-
HJI43_RS03600	742996..743442	-	447	WP_023298965.1	GNAT family N-acetyltransferase	-
HJI43_RS03605	743507..743596	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
HJI43_RS03610	744083..744397	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
HJI43_RS03615	744394..744813	+	420	WP_023305145.1	helix-turn-helix domain-containing protein	Antitoxin
HJI43_RS03620	744960..745058	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
HJI43_RS03625	745165..746853	+	1689	WP_048966211.1	acetolactate synthase large subunit	-
HJI43_RS03630	746857..747144	+	288	WP_045336030.1	acetolactate synthase small subunit	-
HJI43_RS03635	747270..747863	+	594	WP_022646555.1	transcriptional regulator UhpA	-
HJI43_RS03640	747860..749365	+	1506	WP_169440842.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-137)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T155677 WP_022646552.1 NZ_CP052870:744083-744397 [Enterobacter cloacae]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T155677 NZ_CP068811:c1964722-1964348 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15508.15 Da
• Isoelectric Point: 5.6761

>AT155677 WP_023305145.1 NZ_CP052870:744394-744813 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAVRFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT155677 NZ_CP068811:c1965179-1964811 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A155SSD8