Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3072767..3073380 Replicon chromosome
Accession NZ_CP052769
Organism Mesorhizobium japonicum strain R7AstarV2

Toxin (Protein)


Gene name higB Uniprot ID A0A4Q1US98
Locus tag HKB46_RS15050 Protein ID WP_032930829.1
Coordinates 3073084..3073380 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HKB46_RS15045 Protein ID WP_032930830.1
Coordinates 3072767..3073075 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKB46_RS15030 3068630..3069808 - 1179 WP_032930833.1 lytic murein transglycosylase -
HKB46_RS15035 3070223..3071023 - 801 WP_032930832.1 glycoside hydrolase family 25 protein -
HKB46_RS15040 3071312..3072691 + 1380 WP_032930831.1 serine hydrolase -
HKB46_RS15045 3072767..3073075 - 309 WP_032930830.1 HigA family addiction module antidote protein Antitoxin
HKB46_RS15050 3073084..3073380 - 297 WP_032930829.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HKB46_RS31410 3073446..3073640 - 195 WP_080680274.1 hypothetical protein -
HKB46_RS15060 3073693..3074178 - 486 WP_032930828.1 hypothetical protein -
HKB46_RS15065 3074354..3075037 - 684 WP_032930827.1 class I SAM-dependent methyltransferase -
HKB46_RS15070 3075034..3076332 - 1299 WP_032930826.1 DUF3419 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11583.01 Da        Isoelectric Point: 7.3192

>T155586 WP_032930829.1 NZ_CP052769:c3073380-3073084 [Mesorhizobium japonicum]
MIVGFRDGWLRAFFVDDTRSRNIPSDLESRLFRKLQMIDDATVDQDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
GGGRGEASDIYLDDHSYK

Download         Length: 297 bp

>T155586 NZ_CP068808:258444-258551 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11306.05 Da        Isoelectric Point: 9.4051

>AT155586 WP_032930830.1 NZ_CP052769:c3073075-3072767 [Mesorhizobium japonicum]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Download         Length: 309 bp

>AT155586 NZ_CP068808:c258386-258332 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q1US98

Antitoxin

Source ID Structure

References