Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3716298..3716895 Replicon chromosome
Accession NZ_CP052761
Organism Klebsiella pneumoniae strain XH210

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag RJ97_RS18295 Protein ID WP_004142563.1
Coordinates 3716578..3716895 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag RJ97_RS18290 Protein ID WP_004142561.1
Coordinates 3716298..3716585 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJ97_RS18260 (3712378) 3712378..3712626 + 249 WP_002893037.1 DUF1158 domain-containing protein -
RJ97_RS18265 (3712644) 3712644..3712985 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
RJ97_RS18270 (3713016) 3713016..3714131 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
RJ97_RS18275 (3714311) 3714311..3714892 + 582 Protein_3586 TetR/AcrR family transcriptional regulator -
RJ97_RS18280 (3714892) 3714892..3715260 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
RJ97_RS18285 (3715380) 3715380..3716033 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
RJ97_RS18290 (3716298) 3716298..3716585 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
RJ97_RS18295 (3716578) 3716578..3716895 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
RJ97_RS18300 (3717080) 3717080..3718123 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
RJ97_RS18305 (3718792) 3718792..3719658 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
RJ97_RS18310 (3719767) 3719767..3721194 + 1428 WP_023284820.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T155573 WP_004142563.1 NZ_CP052761:c3716895-3716578 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T155573 NZ_CP068807:3647848-3648138 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT155573 WP_004142561.1 NZ_CP052761:c3716585-3716298 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT155573 NZ_CP068807:3647616-3647858 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References