Detailed information of TA system TA155559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5654094..5654689
• Replicon: chromosome
• Accession: NZ_CP052759
• Organism: Pseudomonas aeruginosa strain LYT4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: HH306_RS26680
• Protein ID: WP_003117425.1
• Coordinates: 5654411..5654689 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HH306_RS26675
• Protein ID: WP_003099268.1
• Coordinates: 5654094..5654399 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH306_RS26640	5649233..5650081	+	849	WP_003123430.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HH306_RS26650	5650248..5651189	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
HH306_RS26655	5651306..5651920	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
HH306_RS26660	5651962..5652546	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
HH306_RS26665	5652587..5653687	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
HH306_RS26675	5654094..5654399	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
HH306_RS26680	5654411..5654689	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HH306_RS26685	5655018..5657246	+	2229	WP_003113525.1	TonB-dependent receptor	-
HH306_RS26690	5657316..5657963	-	648	WP_003095021.1	carbonate dehydratase	-
HH306_RS26695	5658025..5659263	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T155559 WP_003117425.1 NZ_CP052759:c5654689-5654411 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T155559 NZ_CP068807:c1686493-1686182 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT155559 WP_003099268.1 NZ_CP052759:c5654399-5654094 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT155559 NZ_CP068807:c1686185-1685766 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5