Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4852805..4853402 Replicon chromosome
Accession NZ_CP052036
Organism Klebsiella pneumoniae strain KPN41053

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag HH204_RS23380 Protein ID WP_004142563.1
Coordinates 4853085..4853402 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag HH204_RS23375 Protein ID WP_004142561.1
Coordinates 4852805..4853092 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HH204_RS23345 4848887..4849135 + 249 WP_002893037.1 DUF1158 domain-containing protein -
HH204_RS23350 4849153..4849494 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
HH204_RS23355 4849525..4850640 - 1116 WP_172676897.1 MBL fold metallo-hydrolase -
HH204_RS23360 4850818..4851399 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
HH204_RS23365 4851399..4851767 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
HH204_RS23370 4851887..4852540 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
HH204_RS23375 4852805..4853092 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
HH204_RS23380 4853085..4853402 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HH204_RS23385 4853557..4854525 - 969 WP_004225014.1 IS5 family transposase -
HH204_RS23390 4854652..4855695 - 1044 WP_088498345.1 DUF2157 domain-containing protein -
HH204_RS23395 4856365..4857231 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4853557..4854480 923


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T155415 WP_004142563.1 NZ_CP052036:c4853402-4853085 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T155415 NZ_CP068801:2043052-2043303 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT155415 WP_004142561.1 NZ_CP052036:c4853092-4852805 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT155415 NZ_CP068801:2043300-2043554 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References