Detailed information of TA system TA155388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 35253..35838
• Replicon: plasmid pAb-C63_1
• Accession: NZ_CP051867
• Organism: Acinetobacter baumannii strain Ab-C63

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: HIN88_RS18675
• Protein ID: WP_000897307.1
• Coordinates: 35253..35573 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIN88_RS18680
• Protein ID: WP_000369781.1
• Coordinates: 35566..35838 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN88_RS18645	31172..31738	+	567	WP_000678311.1	recombinase family protein	-
HIN88_RS18650	31981..33168	-	1188	WP_004856455.1	tetracycline efflux MFS transporter Tet(39)	-
HIN88_RS18655	33234..33875	+	642	WP_004787586.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-
HIN88_RS18660	34030..34332	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN88_RS18665	34333..34614	+	282	WP_000985609.1	putative addiction module antidote protein	-
HIN88_RS18670	34694..35113	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
HIN88_RS18675	35253..35573	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN88_RS18680	35566..35838	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
HIN88_RS18685	36331..37806	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
HIN88_RS18690	37862..38746	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
HIN88_RS18695	38936..39547	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / msr(E) / mph(E) / aph(3')-Ia / ant(2'')-Ia / blaOXA-420 / aph(3'')-Ib / aph(6)-Id / sul2 / dfrA20	-	1..81353	81353
	-	inside	IScluster/Tn	tet(39) / msr(E) / mph(E) / aph(3')-Ia / ant(2'')-Ia / blaOXA-420 / aph(3'')-Ib / aph(6)-Id / sul2 / dfrA20	-	29894..59209	29315

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T155388 WP_000897307.1 NZ_CP051867:35253-35573 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T155388 NZ_CP068800:2334550-2334653 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT155388 WP_000369781.1 NZ_CP051867:35566-35838 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT155388 NZ_CP068800:c2334691-2334420 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3