Detailed information of TA system TA155384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 13515..14100
• Replicon: plasmid pAb-C102_3
• Accession: NZ_CP051865
• Organism: Acinetobacter baumannii strain Ab-C102

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8R027
• Locus tag: HIN89_RS18720
• Protein ID: WP_004698338.1
• Coordinates: 13515..13835 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIN89_RS18725
• Protein ID: WP_000369781.1
• Coordinates: 13828..14100 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN89_RS18700	9761..9952	-	192	WP_000102616.1	hypothetical protein	-
HIN89_RS18705	11359..12288	+	930	WP_005133531.1	replication initiation protein RepM	-
HIN89_RS18710	12367..12891	+	525	WP_005127114.1	plasmid replication DNA-binding protein	-
HIN89_RS18715	13088..13348	-	261	WP_004698336.1	hypothetical protein	-
HIN89_RS18720	13515..13835	+	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN89_RS18725	13828..14100	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
HIN89_RS18730	14190..15515	-	1326	WP_016805832.1	ISNCY family transposase	-
HIN89_RS18735	15665..16063	-	399	WP_024437500.1	hypothetical protein	-
HIN89_RS18740	16085..16498	-	414	WP_086266947.1	DUF3800 domain-containing protein	-
HIN89_RS18745	16562..16807	+	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HIN89_RS18750	16797..17090	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18755	17128..17400	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
HIN89_RS18760	17393..17713	-	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18765	17879..18139	+	261	WP_004698336.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19853	19853
	-	inside	IScluster/Tn	-	-	4260..19635	15375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12222.01 Da
• Isoelectric Point: 6.9800

>T155384 WP_004698338.1 NZ_CP051865:13515-13835 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQEDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLLAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T155384 NZ_CP068800:c904675-904298 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT155384 WP_000369781.1 NZ_CP051865:13828-14100 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT155384 NZ_CP068800:c905096-904722 [Escherichia coli]
GTGTCAGACACACACCCCGGGACAACGCATCCCGACAACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: N8R027