Detailed information of TA system TA155383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 7198..7783
• Replicon: plasmid pAb-C102_3
• Accession: NZ_CP051865
• Organism: Acinetobacter baumannii strain Ab-C102

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8R027
• Locus tag: HIN89_RS18685
• Protein ID: WP_004698338.1
• Coordinates: 7463..7783 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIN89_RS18680
• Protein ID: WP_000369781.1
• Coordinates: 7198..7470 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN89_RS18635	2438..2962	+	525	WP_005127114.1	plasmid replication DNA-binding protein	-
HIN89_RS18640	3159..3419	-	261	WP_004698336.1	hypothetical protein	-
HIN89_RS18645	3586..3906	+	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18650	3899..4171	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	-
HIN89_RS18655	4260..5585	-	1326	WP_016805832.1	ISNCY family transposase	-
HIN89_RS18660	5735..6133	-	399	WP_024437500.1	hypothetical protein	-
HIN89_RS18665	6155..6568	-	414	WP_086266947.1	DUF3800 domain-containing protein	-
HIN89_RS18670	6632..6877	+	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
HIN89_RS18675	6867..7160	+	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HIN89_RS18680	7198..7470	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
HIN89_RS18685	7463..7783	-	321	WP_004698338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN89_RS18690	7950..8210	+	261	WP_004698336.1	hypothetical protein	-
HIN89_RS18695	8381..9706	+	1326	WP_016805832.1	ISNCY family transposase	-
HIN89_RS18700	9761..9952	-	192	WP_000102616.1	hypothetical protein	-
HIN89_RS18705	11359..12288	+	930	WP_005133531.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..19853	19853
	-	inside	IScluster/Tn	-	-	4260..19635	15375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12222.01 Da
• Isoelectric Point: 6.9800

>T155383 WP_004698338.1 NZ_CP051865:c7783-7463 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQEDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLLAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T155383 NZ_CP068800:c830020-829643 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT155383 WP_000369781.1 NZ_CP051865:c7470-7198 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT155383 NZ_CP068800:c830478-830110 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: N8R027