Detailed information of TA system TA155380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 20917..21568
• Replicon: plasmid pAb-C102_1
• Accession: NZ_CP051863
• Organism: Acinetobacter baumannii strain Ab-C102

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BC17
• Locus tag: HIN89_RS17995
• Protein ID: WP_004967307.1
• Coordinates: 21212..21568 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIN89_RS17990
• Protein ID: WP_001140620.1
• Coordinates: 20917..21219 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIN89_RS17970	16124..16663	+	540	WP_002055813.1	hypothetical protein	-
HIN89_RS17975	16706..16966	+	261	Protein_16	transposase	-
HIN89_RS17980	17048..19003	+	1956	WP_000693745.1	MBL fold metallo-hydrolase	-
HIN89_RS17985	19508..20833	+	1326	WP_016805832.1	ISNCY family transposase	-
HIN89_RS17990	20917..21219	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
HIN89_RS17995	21212..21568	-	357	WP_004967307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIN89_RS18005	21789..22625	-	837	WP_000480968.1	aminoglycoside O-phosphotransferase APH(6)-Id	-
HIN89_RS18010	22625..23428	-	804	WP_001082319.1	aminoglycoside O-phosphotransferase APH(3'')-Ib	-
HIN89_RS18015	23504..24436	-	933	WP_086374392.1	IS5 family transposase	-
HIN89_RS18020	24444..24698	-	255	WP_051626581.1	phosphoglucosamine mutase	-
HIN89_RS18025	24785..25600	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / floR / blaOXA-58	-	1..90089	90089
	-	inside	IScluster/Tn	aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-Ia / floR / blaOXA-58	-	16685..47029	30344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13601.62 Da
• Isoelectric Point: 6.4648

>T155380 WP_004967307.1 NZ_CP051863:c21568-21212 [Acinetobacter baumannii]
MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIADKGSKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T155380 NZ_CP051863:c21568-21212 [Acinetobacter baumannii]
ATGTGGATAGTCATTACGACAGATCTATTTAATGAATGGCTTGAACAGCAAGACGAATCAACACAAGAGAAGGTTCTGGC
TGCCCTAGTTGTTCTACAGCAGCAAGGACCAAGTTTAGGCCGCCCTTTAGTAGATACTGTCTATGACTCTAAATTTACCA
ACATGAAAGAGCTTCGTGTTCAACATCGAGGTAAACCACTGAGAGCTTTCTTCGCATTTGATCCTTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCAGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACCACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT155380 WP_001140620.1 NZ_CP051863:c21219-20917 [Acinetobacter baumannii]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT155380 NZ_CP051863:c21219-20917 [Acinetobacter baumannii]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCAGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTTGAAATCTCTCAAAAGGAACTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGCGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAATGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7T4TW67