Detailed information of TA system TA155357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5140254..5140856
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ20_RS24825
• Protein ID: WP_000897302.1
• Coordinates: 5140545..5140856 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ20_RS24820
• Protein ID: WP_000356397.1
• Coordinates: 5140254..5140544 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS24795 (5136327)	5136327..5137229	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ20_RS24800 (5137226)	5137226..5137861	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ20_RS24805 (5137858)	5137858..5138787	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ20_RS24810 (5139003)	5139003..5139221	-	219	WP_001314326.1	CopG family transcriptional regulator	-
HHJ20_RS24815 (5139617)	5139617..5139895	-	279	WP_001296612.1	hypothetical protein	-
HHJ20_RS24820 (5140254)	5140254..5140544	-	291	WP_000356397.1	NadS family protein	Antitoxin
HHJ20_RS24825 (5140545)	5140545..5140856	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ20_RS24830 (5141086)	5141086..5141994	+	909	WP_001305059.1	alpha/beta hydrolase	-
HHJ20_RS24835 (5142058)	5142058..5142999	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ20_RS24840 (5143044)	5143044..5143481	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HHJ20_RS24845 (5143478)	5143478..5144350	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ20_RS24850 (5144344)	5144344..5144943	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T155357 WP_000897302.1 NZ_CP051849:c5140856-5140545 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155357 NZ_CP068799:1129642-1129977 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGA
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155357 WP_000356397.1 NZ_CP051849:c5140544-5140254 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155357 NZ_CP068799:1129394-1129642 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: S1P416