Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2777177..2777739 Replicon chromosome
Accession NZ_CP051849
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HHJ20_RS13405 Protein ID WP_000605675.1
Coordinates 2777177..2777455 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ20_RS13410 Protein ID WP_000781370.1
Coordinates 2777455..2777739 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ20_RS13380 (2772763) 2772763..2773194 - 432 WP_000152307.1 peroxiredoxin OsmC -
HHJ20_RS13385 (2773539) 2773539..2773754 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ20_RS13390 (2773856) 2773856..2773993 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ20_RS13395 (2774150) 2774150..2775847 + 1698 WP_000433460.1 malate dehydrogenase -
HHJ20_RS13400 (2775981) 2775981..2776991 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HHJ20_RS13405 (2777177) 2777177..2777455 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ20_RS13410 (2777455) 2777455..2777739 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
HHJ20_RS13415 (2777968) 2777968..2778621 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HHJ20_RS13420 (2778614) 2778614..2779498 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HHJ20_RS13425 (2779511) 2779511..2782558 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T155344 WP_000605675.1 NZ_CP051849:2777177-2777455 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T155344 NZ_CP068798:796538-796948 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155344 WP_000781370.1 NZ_CP051849:2777455-2777739 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155344 NZ_CP068798:796291-796557 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References