Detailed information of TA system TA155334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2143345..2143847
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: HHJ20_RS10335
• Protein ID: WP_001560606.1
• Coordinates: 2143593..2143847 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: HHJ20_RS10330
• Protein ID: WP_001259255.1
• Coordinates: 2143345..2143596 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS10305 (2138523)	2138523..2139590	-	1068	WP_000080081.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
HHJ20_RS10310 (2139590)	2139590..2140660	-	1071	WP_001560609.1	histidinol-phosphate transaminase	-
HHJ20_RS10315 (2140657)	2140657..2141961	-	1305	WP_001560608.1	histidinol dehydrogenase	-
HHJ20_RS10320 (2141967)	2141967..2142866	-	900	WP_001560607.1	ATP phosphoribosyltransferase	-
HHJ20_RS10325 (2143012)	2143012..2143062	-	51	WP_001364200.1	his operon leader peptide	-
HHJ20_RS10330 (2143345)	2143345..2143596	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HHJ20_RS10335 (2143593)	2143593..2143847	+	255	WP_001560606.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HHJ20_RS10340 (2143930)	2143930..2144754	+	825	WP_000754737.1	SDR family oxidoreductase	-
HHJ20_RS10345 (2144800)	2144800..2145729	+	930	WP_023565113.1	LysR substrate-binding domain-containing protein	-
HHJ20_RS10350 (2145944)	2145944..2146006	+	63	WP_097433929.1	membrane protein YoeI	-
HHJ20_RS10355 (2145996)	2145996..2147354	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
HHJ20_RS10360 (2147533)	2147533..2148591	+	1059	WP_024189617.1	thiosulfate utilization transporter TsuA/YeeE	-
HHJ20_RS10365 (2148605)	2148605..2148832	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10153.60 Da
• Isoelectric Point: 8.8560

>T155334 WP_001560606.1 NZ_CP051849:2143593-2143847 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEVHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T155334 NZ_CP068797:824229-824639 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT155334 WP_001259255.1 NZ_CP051849:2143345-2143596 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT155334 NZ_CP068797:823982-824248 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0