Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1323080..1323807 Replicon chromosome
Accession NZ_CP051849
Organism Escherichia coli strain SCU-101

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HHJ20_RS06365 Protein ID WP_000547555.1
Coordinates 1323080..1323391 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HHJ20_RS06370 Protein ID WP_000126293.1
Coordinates 1323388..1323807 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ20_RS06335 (1318221) 1318221..1319930 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HHJ20_RS06340 (1319940) 1319940..1320482 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HHJ20_RS06345 (1320482) 1320482..1321249 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HHJ20_RS06350 (1321246) 1321246..1321656 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HHJ20_RS06355 (1321649) 1321649..1322119 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HHJ20_RS06360 (1322162) 1322162..1322917 + 756 WP_001305268.1 hypothetical protein -
HHJ20_RS06365 (1323080) 1323080..1323391 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HHJ20_RS06370 (1323388) 1323388..1323807 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HHJ20_RS06375 (1323899) 1323899..1324327 - 429 WP_000536064.1 hypothetical protein -
HHJ20_RS06380 (1324712) 1324712..1325239 + 528 WP_001078782.1 electron transport protein HydN -
HHJ20_RS06385 (1325392) 1325392..1327644 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T155333 WP_000547555.1 NZ_CP051849:1323080-1323391 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T155333 NZ_CP068797:324412-324780 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT155333 WP_000126293.1 NZ_CP051849:1323388-1323807 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT155333 NZ_CP068797:324194-324415 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References