Detailed information of TA system TA155329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 685395..686194
• Replicon: chromosome
• Accession: NZ_CP051849
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: HHJ20_RS03335
• Protein ID: WP_000347252.1
• Coordinates: 685395..685859 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: HHJ20_RS03340
• Protein ID: WP_001296435.1
• Coordinates: 685859..686194 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ20_RS03305 (680396)	680396..680830	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HHJ20_RS03310 (680848)	680848..681726	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
HHJ20_RS03315 (681716)	681716..682495	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HHJ20_RS03320 (682506)	682506..682979	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HHJ20_RS03325 (683002)	683002..684282	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HHJ20_RS03330 (684531)	684531..685340	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HHJ20_RS03335 (685395)	685395..685859	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HHJ20_RS03340 (685859)	685859..686194	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HHJ20_RS03345 (686343)	686343..687914	-	1572	WP_001273940.1	galactarate dehydratase	-
HHJ20_RS03350 (688289)	688289..689623	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
HHJ20_RS03355 (689639)	689639..690409	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T155329 WP_000347252.1 NZ_CP051849:c685859-685395 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T155329 NZ_CP068796:c4577805-4577515 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT155329 WP_001296435.1 NZ_CP051849:c686194-685859 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT155329 NZ_CP068796:c4578037-4577795 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5