Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3072774..3073387 Replicon chromosome
Accession NZ_CP051772
Organism Mesorhizobium japonicum R7A

Toxin (Protein)


Gene name higB Uniprot ID A0A4Q1US98
Locus tag R7A2020_RS15090 Protein ID WP_032930829.1
Coordinates 3073091..3073387 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag R7A2020_RS15085 Protein ID WP_032930830.1
Coordinates 3072774..3073082 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R7A2020_RS15070 3068637..3069815 - 1179 WP_032930833.1 lytic murein transglycosylase -
R7A2020_RS15075 3070230..3071030 - 801 WP_032930832.1 glycoside hydrolase family 25 protein -
R7A2020_RS15080 3071319..3072698 + 1380 WP_032930831.1 serine hydrolase -
R7A2020_RS15085 3072774..3073082 - 309 WP_032930830.1 HigA family addiction module antidote protein Antitoxin
R7A2020_RS15090 3073091..3073387 - 297 WP_032930829.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
R7A2020_RS31560 3073453..3073647 - 195 WP_080680274.1 hypothetical protein -
R7A2020_RS15100 3073700..3074185 - 486 WP_032930828.1 hypothetical protein -
R7A2020_RS15105 3074361..3075044 - 684 WP_032930827.1 class I SAM-dependent methyltransferase -
R7A2020_RS15110 3075041..3076339 - 1299 WP_032930826.1 DUF3419 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-95)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11583.01 Da        Isoelectric Point: 7.3192

>T155325 WP_032930829.1 NZ_CP051772:c3073387-3073091 [Mesorhizobium japonicum R7A]
MIVGFRDGWLRAFFVDDTRSRNIPSDLESRLFRKLQMIDDATVDQDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
GGGRGEASDIYLDDHSYK

Download         Length: 297 bp

>T155325 NZ_CP068796:c4051552-4050632 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11306.05 Da        Isoelectric Point: 9.4051

>AT155325 WP_032930830.1 NZ_CP051772:c3073082-3072774 [Mesorhizobium japonicum R7A]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Download         Length: 309 bp

>AT155325 NZ_CP068796:c4052321-4051539 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q1US98

Antitoxin

Source ID Structure

References