Detailed information of TA system TA155322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4565006..4565608
• Replicon: chromosome
• Accession: NZ_CP051765
• Organism: Escherichia coli strain SCU-113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HHJ30_RS21735
• Protein ID: WP_000897305.1
• Coordinates: 4565297..4565608 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ30_RS21730
• Protein ID: WP_000356397.1
• Coordinates: 4565006..4565296 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ30_RS21705	4560931..4561833	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ30_RS21710	4561830..4562465	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ30_RS21715	4562462..4563391	+	930	WP_000027707.1	formate dehydrogenase accessory protein FdhE	-
HHJ30_RS21720	4563721..4563963	-	243	WP_001087409.1	hypothetical protein	-
HHJ30_RS21725	4564183..4564401	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
HHJ30_RS21730	4565006..4565296	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ30_RS21735	4565297..4565608	-	312	WP_000897305.1	hypothetical protein	Toxin
HHJ30_RS21740	4565837..4566745	+	909	WP_001162704.1	alpha/beta hydrolase	-
HHJ30_RS21745	4566809..4567750	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ30_RS21750	4567795..4568232	-	438	WP_174190320.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ30_RS21755	4568229..4569101	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ30_RS21760	4569095..4569694	-	600	WP_001295269.1	glucose-1-phosphatase	-
HHJ30_RS21765	4569793..4570578	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T155322 WP_000897305.1 NZ_CP051765:c4565608-4565297 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155322 NZ_CP068796:3135935-3136429 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155322 WP_000356397.1 NZ_CP051765:c4565296-4565006 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155322 NZ_CP068796:3135645-3135938 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7