Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1792583..1793085 Replicon chromosome
Accession NZ_CP051765
Organism Escherichia coli strain SCU-113

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HHJ30_RS08435 Protein ID WP_000767829.1
Coordinates 1792831..1793085 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HHJ30_RS08430 Protein ID WP_001259255.1
Coordinates 1792583..1792834 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ30_RS08405 1787761..1788828 - 1068 WP_000080102.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HHJ30_RS08410 1788828..1789898 - 1071 WP_052903468.1 histidinol-phosphate transaminase -
HHJ30_RS08415 1789895..1791199 - 1305 WP_160513582.1 histidinol dehydrogenase -
HHJ30_RS08420 1791205..1792104 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HHJ30_RS08425 1792250..1792300 - 51 WP_001364200.1 his operon leader peptide -
HHJ30_RS08430 1792583..1792834 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HHJ30_RS08435 1792831..1793085 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HHJ30_RS08440 1793168..1793992 + 825 WP_000754763.1 SDR family oxidoreductase -
HHJ30_RS08445 1794038..1794967 + 930 WP_000803351.1 LysR family transcriptional regulator -
HHJ30_RS08450 1795182..1795244 + 63 WP_010723108.1 membrane protein YoeI -
HHJ30_RS08455 1795234..1796592 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HHJ30_RS08460 1796771..1797829 + 1059 WP_000492340.1 transport protein YeeE -
HHJ30_RS08465 1797843..1798070 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T155306 WP_000767829.1 NZ_CP051765:1792831-1793085 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T155306 NZ_CP068795:c4416542-4416369 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT155306 WP_001259255.1 NZ_CP051765:1792583-1792834 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT155306 NZ_CP068795:c4416866-4416570 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References