Detailed information of TA system TA155302

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 609572..610371
• Replicon: chromosome
• Accession: NZ_CP051765
• Organism: Escherichia coli strain SCU-113

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A6H2GMC5
• Locus tag: HHJ30_RS02945
• Protein ID: WP_000347279.1
• Coordinates: 609572..610036 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: HHJ30_RS02950
• Protein ID: WP_001307405.1
• Coordinates: 610036..610371 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ30_RS02915	604573..605007	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
HHJ30_RS02920	605025..605903	-	879	WP_001298758.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HHJ30_RS02925	605893..606672	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HHJ30_RS02930	606683..607156	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HHJ30_RS02935	607179..608459	-	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HHJ30_RS02940	608708..609517	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HHJ30_RS02945	609572..610036	-	465	WP_000347279.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HHJ30_RS02950	610036..610371	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HHJ30_RS02955	610520..612091	-	1572	WP_001273741.1	galactarate dehydratase	-
HHJ30_RS02960	612466..613800	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
HHJ30_RS02965	613816..614586	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	609572..622306	12734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17869.24 Da
• Isoelectric Point: 9.4947

>T155302 WP_000347279.1 NZ_CP051765:c610036-609572 [Escherichia coli]
MDFPQRVNGWVLYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESFTQETEENH

Nucleotide

• Length: 465 bp

>T155302 NZ_CP068795:c3834648-3834250 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT155302 WP_001307405.1 NZ_CP051765:c610371-610036 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT155302 NZ_CP068795:c3834944-3834651 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A6H2GMC5

Antitoxin

• AlphaFold DB: A0A0E0XVC7