Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4008479..4009278 Replicon chromosome
Accession NZ_CP051753
Organism Escherichia coli strain SCU-102

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag HHJ21_RS18860 Protein ID WP_000347273.1
Coordinates 4008814..4009278 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HHJ21_RS18855 Protein ID WP_001307405.1
Coordinates 4008479..4008814 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ21_RS18840 4004264..4005034 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
HHJ21_RS18845 4005050..4006384 - 1335 WP_000599654.1 galactarate/glucarate/glycerate transporter GarP -
HHJ21_RS18850 4006759..4008330 + 1572 WP_001273756.1 galactarate dehydratase -
HHJ21_RS18855 4008479..4008814 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHJ21_RS18860 4008814..4009278 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHJ21_RS18865 4009333..4010142 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
HHJ21_RS18870 4010391..4011671 + 1281 WP_000681942.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHJ21_RS18875 4011694..4012167 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHJ21_RS18880 4012178..4012957 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHJ21_RS18885 4012947..4013825 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHJ21_RS18890 4013843..4014277 + 435 WP_000948814.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3999239..4009278 10039


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T155299 WP_000347273.1 NZ_CP051753:4008814-4009278 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T155299 NZ_CP068795:2742562-2742669 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT155299 WP_001307405.1 NZ_CP051753:4008479-4008814 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT155299 NZ_CP068795:c2742515-2742449 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References