Detailed information of TA system TA155280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 859827..860521
• Replicon: chromosome
• Accession: NZ_CP051753
• Organism: Escherichia coli strain SCU-102

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: HHJ21_RS03925
• Protein ID: WP_048967440.1
• Coordinates: 860123..860521 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: HHJ21_RS03920
• Protein ID: WP_000554755.1
• Coordinates: 859827..860120 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ21_RS03900	855269..856018	+	750	WP_000093948.1	C40 family peptidase	-
HHJ21_RS03905	856180..857919	-	1740	WP_048967441.1	flagellar biosynthesis protein FlhA	-
HHJ21_RS03910	857864..858649	+	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
HHJ21_RS03915	858720..859775	+	1056	WP_001226183.1	DNA polymerase IV	-
HHJ21_RS03920	859827..860120	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ21_RS03925	860123..860521	+	399	WP_048967440.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ21_RS03930	860531..860983	+	453	WP_001059899.1	GNAT family N-acetyltransferase	-
HHJ21_RS03935	861216..861482	+	267	WP_001389960.1	RtcB family protein	-
HHJ21_RS03940	861642..861782	+	141	WP_001366468.1	hypothetical protein	-
HHJ21_RS03945	861839..863296	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
HHJ21_RS03950	863557..864015	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HHJ21_RS03955	864107..865351	+	1245	WP_000189541.1	esterase FrsA	-
-	864611..864691	+	81	NuclAT_7	-	-
-	864611..864691	+	81	NuclAT_7	-	-
-	864611..864691	+	81	NuclAT_7	-	-
-	864611..864691	+	81	NuclAT_7	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15416.86 Da
• Isoelectric Point: 7.3840

>T155280 WP_048967440.1 NZ_CP051753:860123-860521 [Escherichia coli]
MRVFKIKLICLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T155280 NZ_CP051753:860123-860521 [Escherichia coli]
ATGCGGGTATTCAAAATAAAACTTATTTGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT155280 WP_000554755.1 NZ_CP051753:859827-860120 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT155280 NZ_CP051753:859827-860120 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829G9H5