Detailed information of TA system TA155253

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 635225..636024
• Replicon: chromosome
• Accession: NZ_CP051751
• Organism: Escherichia coli strain SCU-301

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: HHJ43_RS03005
• Protein ID: WP_000347273.1
• Coordinates: 635225..635689 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: HHJ43_RS03010
• Protein ID: WP_001308975.1
• Coordinates: 635689..636024 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ43_RS02975	630226..630660	-	435	WP_000948834.1	PTS sugar transporter subunit IIA	-
HHJ43_RS02980	630678..631556	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HHJ43_RS02985	631546..632325	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HHJ43_RS02990	632336..632809	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HHJ43_RS02995	632832..634112	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HHJ43_RS03000	634361..635170	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HHJ43_RS03005	635225..635689	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HHJ43_RS03010	635689..636024	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HHJ43_RS03015	636173..637744	-	1572	WP_096986739.1	galactarate dehydratase	-
HHJ43_RS03020	638119..639453	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HHJ43_RS03025	639469..640239	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T155253 WP_000347273.1 NZ_CP051751:c635689-635225 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T155253 NZ_CP068714:c10131-9817 [Staphylococcus pseudoxylosus]
GTGGCTAAATTGAATGTTACCTTTTCACCACAAGCATTTGATGATTATGAATATTTTCAGATAAAAGATAGAAAGAAGGT
TAAAAGGATGAATCAGTTATTAAAAAGTATCTCTAGAGATGGGACATTAGATGCTATAGGTAAACCTGAAAAGTTAGTTG
GCAATTTTTTTGGATATTACAGTAGACGAATTAACCAAGAACATAGATTGGTATATACAGTAAATGACATAATTTTACTT
ATCGGAAGTGCATATGCTTATATATTTACAGATGGTATAATAATGGAGTCGCCTATCTCTCAGGCGTCAATTTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT155253 WP_001308975.1 NZ_CP051751:c636024-635689 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT155253 NZ_CP068714:c10380-10132 [Staphylococcus pseudoxylosus]
ATGATTGTTAAATCTTATTCATATGTACGTAAAAACTTTAAAGATATGATTAATAAAGTGAATAATGATAGCGATACGAT
AACAATTACAACAAAAGATCGTAATGCAGTTATAATGTCGGAAGATAATTATAATGAAATAATGGAAACACTATACTTGC
AACAAAATCCTGCCAATTCGAAACATCTATCAGAATCTATTGAAAACCTAAAACGTGGTAATGTAAAAGCTAAGGATATT
TTGATATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08