Detailed information of TA system TA155246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4925134..4925736
• Replicon: chromosome
• Accession: NZ_CP051744
• Organism: Escherichia coli strain SCU-484

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ53_RS23565
• Protein ID: WP_000897302.1
• Coordinates: 4925425..4925736 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ53_RS23560
• Protein ID: WP_000356397.1
• Coordinates: 4925134..4925424 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ53_RS23535	4921206..4922108	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ53_RS23540	4922105..4922740	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ53_RS23545	4922737..4923666	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ53_RS23550	4923882..4924100	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ53_RS23555	4924497..4924775	-	279	WP_001296612.1	hypothetical protein	-
HHJ53_RS23560	4925134..4925424	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ53_RS23565	4925425..4925736	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ53_RS23570	4925965..4926873	+	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ53_RS23575	4926937..4927878	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ53_RS23580	4927923..4928360	-	438	WP_000560990.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ53_RS23585	4928357..4929229	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ53_RS23590	4929223..4929822	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T155246 WP_000897302.1 NZ_CP051744:c4925736-4925425 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155246 NZ_CP068710:1329794-1329973 [Burkholderia thailandensis]
ATGAACTCATCGAAGCTGATCCGGATGCTTGAGGAAGATGGCTGGAGGTTGGTTCGAGTGACAGGCAGCCATCATCATTT
CAAACACCCCAAGAAGCCAGGTCTTGTGACGGTTCCCCACCCGAAGAAGGACCTACCGATTGGGACTGTAAAAAGCATCC
AGAAATCCGCCGGCCTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155246 WP_000356397.1 NZ_CP051744:c4925424-4925134 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155246 NZ_CP068710:1330017-1330433 [Burkholderia thailandensis]
ATGGAATTTCCCATCGCAGTGCATAAGGACGATGGGAGCGTCTACGGCGTGACGGTCCCGGATATTCCGGGCGTTCACTC
CTGGGGCGAAACCATCGATGATGCAATCAAAAACACGAGAGAGGCTATCGTCGGCCATGTAGAGACATTGATCGAGCTTG
GGGACGATGTTGAATTCACTTGCTCGACGGTGGAAGAACTGGTTGCAAAGCCCGAGTACGCCGGGGCGGTTTGGGCGCTC
GTCAGCGTTGATCTTTCTCAGCTTGATTCCAAGCCCGAGCGGATCAATGTGAGTATCCCTCGCTTCGTGCTGCACAAGAT
TGACGCCTATGTGGCATCCCGCCACGAGACTAGAAGCGGATTTCTTGCGCGTGCTGCCCTGGAGGCACTTAATGAAGGCA
AAGTTAGGCACGCATAA

Structures

Toxin

• AlphaFold DB: S1P416