Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3883282..3883976 Replicon chromosome
Accession NZ_CP051744
Organism Escherichia coli strain SCU-484

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag HHJ53_RS18600 Protein ID WP_001263501.1
Coordinates 3883282..3883680 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag HHJ53_RS18605 Protein ID WP_000554755.1
Coordinates 3883683..3883976 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ53_RS18575 3878647..3879105 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
HHJ53_RS18580 3879366..3880823 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
HHJ53_RS18585 3880880..3881494 - 615 WP_000602123.1 peptide chain release factor H -
HHJ53_RS18590 3881491..3882630 - 1140 WP_000521578.1 RNA ligase RtcB family protein -
HHJ53_RS18595 3882820..3883272 - 453 WP_001059897.1 GNAT family N-acetyltransferase -
HHJ53_RS18600 3883282..3883680 - 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HHJ53_RS18605 3883683..3883976 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HHJ53_RS18610 3884028..3885080 - 1053 WP_000691316.1 DNA polymerase IV -
HHJ53_RS18615 3885273..3886182 - 910 Protein_3639 helix-turn-helix domain-containing protein -
HHJ53_RS18620 3886773..3887066 + 294 WP_000896590.1 hypothetical protein -
HHJ53_RS18625 3887435..3888007 + 573 WP_000086540.1 recombinase family protein -
HHJ53_RS18630 3888011..3888973 - 963 WP_000231497.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / vat 3830000..3890555 60555


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T155238 WP_001263501.1 NZ_CP051744:c3883680-3883282 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T155238 NZ_CP068707:178158-178601 [Escherichia coli]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT155238 WP_000554755.1 NZ_CP051744:c3883976-3883683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT155238 NZ_CP068707:177931-178161 [Escherichia coli]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References