Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2507302..2507855 Replicon chromosome
Accession NZ_CP051744
Organism Escherichia coli strain SCU-484

Toxin (Protein)


Gene name higB Uniprot ID A0A0G9FUJ7
Locus tag HHJ53_RS11740 Protein ID WP_000605680.1
Coordinates 2507302..2507571 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ53_RS11745 Protein ID WP_000781370.1
Coordinates 2507571..2507855 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ53_RS11715 2502888..2503319 - 432 WP_000152305.1 peroxiredoxin OsmC -
HHJ53_RS11720 2503664..2503879 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ53_RS11725 2503981..2504118 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ53_RS11730 2504275..2505972 + 1698 WP_000433464.1 malate dehydrogenase -
HHJ53_RS11735 2506106..2507116 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HHJ53_RS11740 2507302..2507571 + 270 WP_000605680.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ53_RS11745 2507571..2507855 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ53_RS11750 2508084..2508737 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HHJ53_RS11755 2508730..2509614 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HHJ53_RS11760 2509627..2512674 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-89)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10181.73 Da        Isoelectric Point: 6.8582

>T155235 WP_000605680.1 NZ_CP051744:2507302-2507571 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDA
YILDYEDYH

Download         Length: 270 bp

>T155235 NZ_CP068706:c4254449-4254276 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155235 WP_000781370.1 NZ_CP051744:2507571-2507855 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155235 NZ_CP068706:c4254773-4254477 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FUJ7

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References