Detailed information of TA system TA155215

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81421..81834
• Replicon: plasmid pSCU-105-1
• Accession: NZ_CP051739
• Organism: Escherichia coli strain SCU-105

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A2H4TK50
• Locus tag: HHJ23_RS25135
• Protein ID: WP_000775237.1
• Coordinates: 81421..81582 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81635..81834 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ23_RS25100	76514..76741	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
HHJ23_RS25105	77100..77493	-	394	Protein_92	PAS domain-containing protein	-
HHJ23_RS25110	77685..78066	-	382	Protein_93	relaxosome protein TraM	-
HHJ23_RS25115	78409..78999	+	591	WP_000252684.1	transglycosylase SLT domain-containing protein	-
HHJ23_RS25120	79295..80116	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HHJ23_RS25125	80235..80522	-	288	WP_001595289.1	hypothetical protein	-
HHJ23_RS25920	80823..81120	+	298	Protein_97	hypothetical protein	-
HHJ23_RS25135	81421..81582	-	162	WP_000775237.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	81635..81834	-	200	NuclAT_0	-	Antitoxin
-	81635..81834	-	200	NuclAT_0	-	Antitoxin
-	81635..81834	-	200	NuclAT_0	-	Antitoxin
-	81635..81834	-	200	NuclAT_0	-	Antitoxin
HHJ23_RS25140	81791..82105	-	315	WP_000870669.1	hypothetical protein	-
HHJ23_RS25145	82102..82821	-	720	WP_000994097.1	plasmid SOS inhibition protein A	-
HHJ23_RS25150	82818..83252	-	435	WP_000845950.1	conjugation system SOS inhibitor PsiB	-
HHJ23_RS25155	83307..85265	-	1959	WP_001145497.1	ParB/RepB/Spo0J family partition protein	-
HHJ23_RS25160	85324..85557	-	234	WP_000005975.1	DUF905 domain-containing protein	-
HHJ23_RS25165	85613..86140	-	528	WP_000290816.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id	faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..172627	172627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5946.16 Da
• Isoelectric Point: 8.5101

>T155215 WP_000775237.1 NZ_CP051739:c81582-81421 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESGGK

Nucleotide

• Length: 162 bp

>T155215 NZ_CP068706:871931-872266 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 200 bp

>AT155215 NZ_CP051739:c81834-81635 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGGATATGAGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAAAGCCCCGT
AGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A2H4TK50

Antitoxin