Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 654769..655568 Replicon chromosome
Accession NZ_CP051738
Organism Escherichia coli strain SCU-105

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag HHJ23_RS03225 Protein ID WP_000347269.1
Coordinates 654769..655233 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HHJ23_RS03230 Protein ID WP_001307405.1
Coordinates 655233..655568 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ23_RS03195 649770..650204 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HHJ23_RS03200 650222..651100 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHJ23_RS03205 651090..651869 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHJ23_RS03210 651880..652353 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHJ23_RS03215 652376..653656 - 1281 WP_000681955.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHJ23_RS03220 653905..654714 + 810 WP_169063106.1 aga operon transcriptional regulator AgaR -
HHJ23_RS03225 654769..655233 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHJ23_RS03230 655233..655568 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHJ23_RS03235 655717..657288 - 1572 WP_001273738.1 galactarate dehydratase -
HHJ23_RS03240 657663..658997 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HHJ23_RS03245 659013..659783 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T155188 WP_000347269.1 NZ_CP051738:c655233-654769 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T155188 NZ_CP068690:c290797-290327 [Klebsiella pneumoniae subsp. pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT155188 WP_001307405.1 NZ_CP051738:c655568-655233 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT155188 NZ_CP068690:c291237-290794 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References