Detailed information of TA system TA155185

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70528..70781
• Replicon: plasmid pSCU-108-2
• Accession: NZ_CP051737
• Organism: Escherichia coli strain SCU-108

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: HHJ26_RS25585
• Protein ID: WP_001336447.1
• Coordinates: 70632..70781 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70528..70584 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ26_RS25555	66440..67186	+	747	WP_019842127.1	conjugal transfer pilus acetylase TraX	-
HHJ26_RS25560	67241..67798	+	558	WP_000139329.1	fertility inhibition protein FinO	-
HHJ26_RS25565	67950..68153	+	204	WP_001336517.1	hypothetical protein	-
HHJ26_RS25570	68895..69356	+	462	WP_019842128.1	thermonuclease family protein	-
HHJ26_RS25695	69459..69843	+	385	Protein_84	hypothetical protein	-
HHJ26_RS25580	69996..70433	+	438	WP_000872609.1	hypothetical protein	-
-	70528..70584	-	57	NuclAT_1	-	Antitoxin
-	70528..70584	-	57	NuclAT_1	-	Antitoxin
-	70528..70584	-	57	NuclAT_1	-	Antitoxin
-	70528..70584	-	57	NuclAT_1	-	Antitoxin
HHJ26_RS25585	70632..70781	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ26_RS25590	71066..71323	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..71625	71625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T155185 WP_001336447.1 NZ_CP051737:70632-70781 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T155185 NZ_CP068690:c104782-104498 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT155185 NZ_CP051737:c70584-70528 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin