Detailed information of TA system TA155181

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30301..30571
• Replicon: plasmid pSCU-108-2
• Accession: NZ_CP051737
• Organism: Escherichia coli strain SCU-108

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ26_RS25345
• Protein ID: WP_001312861.1
• Coordinates: 30413..30571 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30301..30364 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ26_RS25310	25344..25679	-	336	Protein_32	pilus protein	-
HHJ26_RS25315	26071..26598	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HHJ26_RS25320	26656..26889	+	234	WP_000006003.1	DUF905 family protein	-
HHJ26_RS25325	26950..28914	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
HHJ26_RS25330	28983..29417	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HHJ26_RS25335	29414..30133	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	30145..30369	+	225	NuclAT_0	-	-
-	30145..30369	+	225	NuclAT_0	-	-
-	30145..30369	+	225	NuclAT_0	-	-
-	30145..30369	+	225	NuclAT_0	-	-
HHJ26_RS25340	30154..30333	-	180	WP_001309233.1	hypothetical protein	-
-	30301..30364	-	64	-	-	Antitoxin
HHJ26_RS25345	30413..30571	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HHJ26_RS25350	30808..31143	-	336	WP_032195784.1	hypothetical protein	-
HHJ26_RS25360	31486..31782	+	297	WP_001272251.1	hypothetical protein	-
HHJ26_RS25365	31893..32714	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HHJ26_RS25370	33011..33613	-	603	Protein_43	transglycosylase SLT domain-containing protein	-
HHJ26_RS25375	33934..34317	+	384	WP_001151538.1	relaxosome protein TraM	-
HHJ26_RS25380	34504..35193	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	-	1..71625	71625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T155181 WP_001312861.1 NZ_CP051737:30413-30571 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T155181 NZ_CP068689:c4780092-4779559 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 64 bp

>AT155181 NZ_CP051737:c30364-30301 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin