Detailed information of TA system TA155174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3976103..3976797
• Replicon: chromosome
• Accession: NZ_CP051735
• Organism: Escherichia coli strain SCU-108

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HHJ26_RS18975
• Protein ID: WP_001263500.1
• Coordinates: 3976399..3976797 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HHJ26_RS18970
• Protein ID: WP_000554758.1
• Coordinates: 3976103..3976396 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ26_RS18945	3971639..3972136	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
HHJ26_RS18950	3972219..3972377	-	159	WP_014639450.1	hypothetical protein	-
HHJ26_RS18955	3972456..3974168	-	1713	Protein_3698	flagellar biosynthesis protein FlhA	-
HHJ26_RS18960	3974140..3974925	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
HHJ26_RS18965	3974996..3976051	+	1056	WP_001226168.1	DNA polymerase IV	-
HHJ26_RS18970	3976103..3976396	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HHJ26_RS18975	3976399..3976797	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HHJ26_RS18980	3976807..3977259	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HHJ26_RS18985	3977449..3978588	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
HHJ26_RS18990	3978585..3979199	+	615	WP_000602123.1	peptide chain release factor H	-
HHJ26_RS18995	3979256..3980713	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HHJ26_RS19000	3980974..3981432	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T155174 WP_001263500.1 NZ_CP051735:3976399-3976797 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T155174 NZ_CP068689:366412-366780 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT155174 WP_000554758.1 NZ_CP051735:3976103-3976396 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT155174 NZ_CP068689:366194-366415 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3