Detailed information of TA system TA155149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5000315..5000917
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HHJ27_RS23560
• Protein ID: WP_000897305.1
• Coordinates: 5000315..5000626 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ27_RS23565
• Protein ID: WP_000356395.1
• Coordinates: 5000627..5000917 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS23540	4995357..4995794	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ27_RS23545	4995839..4996780	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ27_RS23550	4997196..4999010	+	1815	WP_001550352.1	hypothetical protein	-
HHJ27_RS23555	4999178..5000086	-	909	WP_001550353.1	alpha/beta hydrolase	-
HHJ27_RS23560	5000315..5000626	+	312	WP_000897305.1	hypothetical protein	Toxin
HHJ27_RS23565	5000627..5000917	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ27_RS23570	5001002..5001223	+	222	WP_001550354.1	hypothetical protein	-
HHJ27_RS23575	5001275..5001553	+	279	WP_001315112.1	hypothetical protein	-
HHJ27_RS23580	5001972..5002190	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
HHJ27_RS23585	5002409..5002651	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
HHJ27_RS23590	5002833..5003762	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ27_RS23595	5003759..5004394	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ27_RS23600	5004391..5005293	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T155149 WP_000897305.1 NZ_CP051733:5000315-5000626 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155149 NZ_CP068682:1287487-1287639 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT155149 WP_000356395.1 NZ_CP051733:5000627-5000917 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155149 NZ_CP068682:1286863-1287462 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAGCATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATTGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCATAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7