Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4164220..4165019 Replicon chromosome
Accession NZ_CP051733
Organism Escherichia coli strain SCU-109

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag HHJ27_RS19625 Protein ID WP_000347270.1
Coordinates 4164555..4165019 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag HHJ27_RS19620 Protein ID WP_001551693.1
Coordinates 4164220..4164555 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ27_RS19605 4160005..4160775 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
HHJ27_RS19610 4160791..4162125 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HHJ27_RS19615 4162500..4164071 + 1572 WP_001273738.1 galactarate dehydratase -
HHJ27_RS19620 4164220..4164555 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HHJ27_RS19625 4164555..4165019 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HHJ27_RS19630 4165074..4165883 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HHJ27_RS19635 4166132..4167412 + 1281 WP_169057754.1 tagatose-bisphosphate aldolase subunit KbaZ -
HHJ27_RS19640 4167435..4167908 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HHJ27_RS19645 4167919..4168698 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HHJ27_RS19650 4168688..4169566 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HHJ27_RS19655 4169584..4170018 + 435 WP_000948837.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4155458..4165019 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T155148 WP_000347270.1 NZ_CP051733:4164555-4165019 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

>T155148 NZ_CP068682:1135030-1135392 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT155148 WP_001551693.1 NZ_CP051733:4164220-4164555 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT155148 NZ_CP068682:1134863-1135033 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References