Detailed information of TA system TA155144

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3635968..3636695
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: HHJ27_RS17145
• Protein ID: WP_000547555.1
• Coordinates: 3636384..3636695 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ27_RS17140
• Protein ID: WP_000126294.1
• Coordinates: 3635968..3636387 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS17120	3631053..3631580	-	528	WP_001078777.1	electron transport protein HydN	-
HHJ27_RS17125	3631729..3632739	-	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
HHJ27_RS17130	3632999..3634456	+	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HHJ27_RS17135	3634465..3635889	+	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
HHJ27_RS17140	3635968..3636387	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ27_RS17145	3636384..3636695	-	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
HHJ27_RS17150	3636862..3637332	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HHJ27_RS17155	3637325..3637735	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HHJ27_RS17160	3637732..3638499	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HHJ27_RS17165	3638499..3639041	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HHJ27_RS17170	3639051..3640760	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T155144 WP_000547555.1 NZ_CP051733:c3636695-3636384 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T155144 NZ_CP068680:4415165-4415707 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACTCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT155144 WP_000126294.1 NZ_CP051733:c3636387-3635968 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT155144 NZ_CP068680:4414870-4415181 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: V0YLE2