Detailed information of TA system TA155129

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2112623..2112994
• Replicon: chromosome
• Accession: NZ_CP051733
• Organism: Escherichia coli strain SCU-109

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HHJ27_RS10020
• Protein ID: WP_001317028.1
• Coordinates: 2112800..2112994 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2112623..2112801 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ27_RS09995	2108578..2109951	+	1374	WP_001551034.1	ATP-dependent RNA helicase DbpA	-
HHJ27_RS10000	2110080..2111015	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HHJ27_RS10005	2111067..2112302	-	1236	WP_000040839.1	site-specific integrase	-
HHJ27_RS10010	2112304..2112519	-	216	WP_000079604.1	excisionase XisR	-
HHJ27_RS10015	2112619..2112807	-	189	WP_001595656.1	DUF1187 family protein	-
-	2112623..2112801	+	179	NuclAT_0	-	Antitoxin
-	2112623..2112801	+	179	NuclAT_0	-	Antitoxin
-	2112623..2112801	+	179	NuclAT_0	-	Antitoxin
-	2112623..2112801	+	179	NuclAT_0	-	Antitoxin
HHJ27_RS10020	2112800..2112994	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HHJ27_RS10025	2113051..2113860	-	810	WP_001595658.1	recombination protein RecT	-
HHJ27_RS10030	2113853..2116453	-	2601	WP_001595659.1	exodeoxyribonuclease VIII	-
HHJ27_RS10035	2116555..2116830	-	276	WP_001314664.1	hypothetical protein	-
HHJ27_RS10040	2116905..2117081	-	177	WP_000245522.1	hypothetical protein	-
HHJ27_RS10045	2117075..2117296	-	222	WP_000560211.1	killing protein KilR	-
HHJ27_RS10050	2117707..2117859	-	153	WP_001169150.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2111067..2153051	41984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T155129 WP_001317028.1 NZ_CP051733:c2112994-2112800 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T155129 NZ_CP068680:1773932-1774246 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT155129 NZ_CP051733:2112623-2112801 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTAGTTAGTTTATATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTAGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin