Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 928834..929528 Replicon chromosome
Accession NZ_CP051733
Organism Escherichia coli strain SCU-109

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag HHJ27_RS04390 Protein ID WP_001263491.1
Coordinates 929130..929528 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag HHJ27_RS04385 Protein ID WP_000554755.1
Coordinates 928834..929127 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ27_RS04360 924455..924811 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HHJ27_RS04365 924804..925082 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HHJ27_RS04370 925187..926899 - 1713 Protein_840 flagellar biosynthesis protein FlhA -
HHJ27_RS04375 926871..927656 + 786 WP_074157994.1 putative lateral flagellar export/assembly protein LafU -
HHJ27_RS04380 927727..928782 + 1056 WP_001550655.1 DNA polymerase IV -
HHJ27_RS04385 928834..929127 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
HHJ27_RS04390 929130..929528 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
HHJ27_RS04395 929538..929990 + 453 WP_019842500.1 GNAT family N-acetyltransferase -
HHJ27_RS04400 930236..930442 + 207 Protein_846 RtcB family protein -
HHJ27_RS24455 930438..930790 + 353 Protein_847 peptide chain release factor H -
HHJ27_RS04410 930847..932304 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
HHJ27_RS04415 932565..933023 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
HHJ27_RS04420 933115..934359 + 1245 WP_000189541.1 esterase FrsA -
- 933619..933699 + 81 NuclAT_10 - -
- 933619..933699 + 81 NuclAT_10 - -
- 933619..933699 + 81 NuclAT_10 - -
- 933619..933699 + 81 NuclAT_10 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T155124 WP_001263491.1 NZ_CP051733:929130-929528 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T155124 NZ_CP068680:c611869-611696 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT155124 WP_000554755.1 NZ_CP051733:928834-929127 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT155124 NZ_CP068680:c612193-611897 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References