Detailed information of TA system TA155105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4601063..4601665
• Replicon: chromosome
• Accession: NZ_CP051727
• Organism: Escherichia coli strain SCU-111

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ28_RS21830
• Protein ID: WP_000897302.1
• Coordinates: 4601354..4601665 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ28_RS21825
• Protein ID: WP_000356397.1
• Coordinates: 4601063..4601353 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ28_RS21800	4597128..4598030	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ28_RS21805	4598027..4598662	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ28_RS21810	4598659..4599588	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ28_RS21815	4599812..4600030	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ28_RS21820	4600426..4600704	-	279	WP_001296612.1	hypothetical protein	-
HHJ28_RS21825	4601063..4601353	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ28_RS21830	4601354..4601665	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ28_RS21835	4601894..4602802	+	909	WP_001331553.1	alpha/beta hydrolase	-
HHJ28_RS21840	4602866..4603807	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ28_RS21845	4603852..4604289	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ28_RS21850	4604286..4605158	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ28_RS21855	4605152..4605751	-	600	WP_001314324.1	glucose-1-phosphatase	-
HHJ28_RS21860	4605850..4606635	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T155105 WP_000897302.1 NZ_CP051727:c4601665-4601354 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155105 NZ_CP068647:c1888908-1888654 [Vibrio parahaemolyticus]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTCAAAGCTGTTTTAGACGGTGTAGTTAATGATGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAGGATGCAGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACTGTTCATT
TACTACGCTCTCCTCAAAACGTTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAGCACGAGAG
TTAATTGATGAGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155105 WP_000356397.1 NZ_CP051727:c4601353-4601063 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155105 NZ_CP068647:c1888661-1888392 [Vibrio parahaemolyticus]
ATGAGTAGTAGTCAACGTTTATTATCGTGGACTGATGATGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAATAAACTCATCAATGATGTTAAGCGCTCTCCATTTGAGGGCATTGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTATTGATGATACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATTTCATGTCGTTACCACTACTAA

Structures

Toxin

• AlphaFold DB: S1P416