Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3382197..3382876 Replicon chromosome
Accession NZ_CP051727
Organism Escherichia coli strain SCU-111

Toxin (Protein)


Gene name higB Uniprot ID A0A777RS26
Locus tag HHJ28_RS16245 Protein ID WP_001764312.1
Coordinates 3382574..3382876 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HHJ28_RS16240 Protein ID WP_000806442.1
Coordinates 3382197..3382538 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ28_RS16230 3378441..3379373 - 933 WP_169062045.1 glutaminase A -
HHJ28_RS16235 3379635..3382139 + 2505 WP_001468080.1 copper-exporting P-type ATPase CopA -
HHJ28_RS16240 3382197..3382538 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HHJ28_RS16245 3382574..3382876 - 303 WP_001764312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ28_RS16250 3383009..3383803 + 795 WP_000365160.1 TraB/GumN family protein -
HHJ28_RS16255 3384007..3384486 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HHJ28_RS16260 3384523..3386175 - 1653 WP_032286904.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HHJ28_RS16265 3386393..3387613 + 1221 WP_001764310.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.3980

>T155096 WP_001764312.1 NZ_CP051727:c3382876-3382574 [Escherichia coli]
MTQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T155096 NZ_CP068633:2795218-2795724 [Vibrio parahaemolyticus]
TTGGGAATAAGTGCACCAACCCTCCTCACTGATGAGCACCAGCTTAATGCCTTTCAATGTGGCATTGAAGAGCTCGATAC
TTGGTTAAGAAAACAGGCGTTAAAGAGTCAAAAGCGGGGAACCGCGAGGGTGTATGTGGTCAATGACACTGACGCCCATC
AAGTGGTCGGGTACTATGCCATTGCCATGGGATCAGTTTCCAGAGAGCAAGCTTTCAGTTCATTAAGAAGAAATAGTCCA
GATCCCATTCCCATGGTGATTCTAGCCAGGCTTGGAGTAGATAACGCCTATCAAGGTCAAGGCATTGCAGCAGGTCTTTT
AAAAGATTGTATCATTCGCTCAGTACAAGCAATGAACGCTGTCGGTGGAGCAGGAATCTTAGTCCATGCTATTGATGGTA
GTGCGCAAACGTTCTACAAGAAATTTGGCTTCAAAGAATCGACGTTTGATCCTTTAGTACTCATGGCAAGGATCTGCGAT
ATCGAAAAATCATTGTCGATAGACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT155096 WP_000806442.1 NZ_CP051727:c3382538-3382197 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT155096 NZ_CP068633:2794961-2795227 [Vibrio parahaemolyticus]
ATGGCTACAAAGACAGCCCCAATCAACATGCGAGTACTGCCATCGGTAAGAGACATCATCGATGCCGCAGCAAGTTTAAA
AAAAGTTGATAGAACCGTGTTCATTCAGCAAGCAGCGCTCAATGAAGCGCATAGTATACTGGCAGAACAGCGGGATTTTG
TTCTAGAAGCAAAGGCTTTTGAAGCGTTTGATAACGAACTTCGTGCAGAGCCACAGACTCTTGAAGGTATGAAAGATCTG
TTTAAGAGGAAAGCGCCTTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A777RS26

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References