Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2402682..2403244 Replicon chromosome
Accession NZ_CP051727
Organism Escherichia coli strain SCU-111

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HHJ28_RS11505 Protein ID WP_000605675.1
Coordinates 2402682..2402960 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HHJ28_RS11510 Protein ID WP_000781370.1
Coordinates 2402960..2403244 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HHJ28_RS11480 2398268..2398699 - 432 WP_000152305.1 peroxiredoxin OsmC -
HHJ28_RS11485 2399044..2399259 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HHJ28_RS11490 2399361..2399498 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HHJ28_RS11495 2399655..2401352 + 1698 WP_000433460.1 malate dehydrogenase -
HHJ28_RS11500 2401486..2402496 + 1011 WP_169062039.1 alcohol dehydrogenase AdhP -
HHJ28_RS11505 2402682..2402960 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HHJ28_RS11510 2402960..2403244 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HHJ28_RS11515 2403431..2404779 + 1349 WP_105457870.1 IS3-like element IS1397 family transposase -
HHJ28_RS11520 2404909..2405562 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HHJ28_RS11525 2405555..2406439 - 885 WP_001764521.1 formate dehydrogenase N subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - espR1 2402682..2414719 12037


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T155094 WP_000605675.1 NZ_CP051727:2402682-2402960 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T155094 NZ_CP068633:2030122-2030400 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAAATCTTCGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAGAAAACTGACAACCTCATTGAAGCAAAAGTAGAAAACTTGCTTGAACAACCTTTAATGGGTGTACAACGAGATGGCA
TCCGCGGACGATTGCTCATTATTCCTGAGATTTCGATGATTGTCTCTTACTGGGTCGAGGGCGATATCATCCGAGTTATG
CGTGTACTCCACCAAAAACAAAAATTCCCTACGGATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT155094 WP_000781370.1 NZ_CP051727:2402960-2403244 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT155094 NZ_CP068633:2029841-2030125 [Vibrio parahaemolyticus]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACAAAACGCCTAGCTCAACAAATGGCTGAGAGCCAAGGTCGCAC
TCTGAGTGACGCATGCCGTGAACTTACTGAGCAACTTGCTGAACAACAAAGAAAAACATTATCTCACGATGCATGGCTAA
CTGAACAAGTAAACCTAGCATTTGAGAAGTTTGACTCAGGAAAGTCCGTTTTCGTTGAGCACCAAACTGCTAAATCTCGA
ATGGAAGAGCGCAAAGCCAGAATCCGCAATCGAGGTAAGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References