Detailed information of TA system TA155076

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84920..85346
• Replicon: plasmid pSCU-112-1
• Accession: NZ_CP051726
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HHJ29_RS24655
• Protein ID: WP_001312861.1
• Coordinates: 84920..85078 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	85122..85346 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS24620	80317..81006	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
HHJ29_RS24625	81193..81576	-	384	WP_001151538.1	relaxosome protein TraM	-
HHJ29_RS24630	81897..82499	+	603	Protein_93	transglycosylase SLT domain-containing protein	-
HHJ29_RS24635	82796..83617	-	822	WP_001234445.1	DUF945 domain-containing protein	-
HHJ29_RS24640	83727..84023	-	297	WP_001272251.1	hypothetical protein	-
HHJ29_RS24650	84415..84619	+	205	Protein_96	pilus protein	-
HHJ29_RS24655	84920..85078	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	85122..85346	-	225	NuclAT_0	-	Antitoxin
-	85122..85346	-	225	NuclAT_0	-	Antitoxin
-	85122..85346	-	225	NuclAT_0	-	Antitoxin
-	85122..85346	-	225	NuclAT_0	-	Antitoxin
HHJ29_RS24660	85158..85337	+	180	WP_001309233.1	hypothetical protein	-
HHJ29_RS24665	85358..86077	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
HHJ29_RS24670	86074..86508	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HHJ29_RS24675	86577..88541	-	1965	WP_001537566.1	ParB/RepB/Spo0J family partition protein	-
HHJ29_RS24680	88602..88835	-	234	WP_000006003.1	DUF905 family protein	-
HHJ29_RS24685	88893..89420	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
HHJ29_RS24690	89722..90159	+	438	WP_024185955.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-48	senB	1..103780	103780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T155076 WP_001312861.1 NZ_CP051726:c85078-84920 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T155076 NZ_CP068622:1899226-1899504 [Vibrio parahaemolyticus]
ATGATTTTATGGGAAGAAGAGTCACTTAATGATCGTGAAAAGATCTTTGAGTTTCTCTATGACTTCAACCCTGATGCGGC
AGAGAAAACTGACAACCTCATTGAAGCAAAAGTGGAGAACTTGCTAGAGCAGCCACTAATGGGTGTGCAGCGAGATGGTA
TTCGTGGGCGATTACTTATAATCCCTGATATTTCAATGATCGTCTCCTATTGGATCGAAGGCGACATTATCCGAATCATG
CGCGTGCTTCACCAGAAACAAAAATTTCCTATGGATTGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT155076 NZ_CP051726:c85346-85122 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin