Detailed information of TA system TA155072

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52990..53243
• Replicon: plasmid pSCU-112-1
• Accession: NZ_CP051726
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: HHJ29_RS24460
• Protein ID: WP_001336447.1
• Coordinates: 52990..53139 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53187..53243 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS24425	49014..49415	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HHJ29_RS24430	49348..49605	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HHJ29_RS24435	49698..50351	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HHJ29_RS24440	50449..50589	-	141	WP_001333237.1	hypothetical protein	-
HHJ29_RS24445	51290..52147	-	858	WP_001537577.1	incFII family plasmid replication initiator RepA	-
HHJ29_RS24450	52140..52214	-	75	WP_001365705.1	RepA leader peptide Tap	-
HHJ29_RS24455	52448..52705	-	258	WP_000084404.1	replication regulatory protein RepA	-
HHJ29_RS24460	52990..53139	-	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	53187..53243	+	57	NuclAT_1	-	Antitoxin
-	53187..53243	+	57	NuclAT_1	-	Antitoxin
-	53187..53243	+	57	NuclAT_1	-	Antitoxin
-	53187..53243	+	57	NuclAT_1	-	Antitoxin
HHJ29_RS24465	53338..53775	-	438	WP_000872609.1	hypothetical protein	-
HHJ29_RS24470	53928..54311	-	384	WP_001109264.1	hypothetical protein	-
HHJ29_RS24475	54414..54875	-	462	WP_000760080.1	thermonuclease family protein	-
HHJ29_RS24480	55628..55831	-	204	WP_001336517.1	hypothetical protein	-
HHJ29_RS24485	55983..56540	-	558	WP_000139329.1	fertility inhibition protein FinO	-
HHJ29_RS24490	56595..57341	-	747	WP_000205709.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-48	senB	1..103780	103780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T155072 WP_001336447.1 NZ_CP051726:c53139-52990 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T155072 NZ_CP068620:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Nucleotide

• Length: 57 bp

>AT155072 NZ_CP051726:53187-53243 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin