Detailed information of TA system TA155071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4877867..4878469
• Replicon: chromosome
• Accession: NZ_CP051725
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HHJ29_RS23140
• Protein ID: WP_000897302.1
• Coordinates: 4878158..4878469 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HHJ29_RS23135
• Protein ID: WP_000356397.1
• Coordinates: 4877867..4878157 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS23110	4873940..4874842	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HHJ29_RS23115	4874839..4875474	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HHJ29_RS23120	4875471..4876400	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HHJ29_RS23125	4876616..4876834	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HHJ29_RS23130	4877230..4877508	-	279	WP_001296612.1	hypothetical protein	-
HHJ29_RS23135	4877867..4878157	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HHJ29_RS23140	4878158..4878469	-	312	WP_000897302.1	hypothetical protein	Toxin
HHJ29_RS23145	4878699..4879607	+	909	WP_001305059.1	alpha/beta hydrolase	-
HHJ29_RS23150	4879671..4880612	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HHJ29_RS23155	4880657..4881094	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HHJ29_RS23160	4881091..4881963	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HHJ29_RS23165	4881957..4882556	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T155071 WP_000897302.1 NZ_CP051725:c4878469-4878158 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T155071 NZ_CP068618:c5277181-5276798 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT155071 WP_000356397.1 NZ_CP051725:c4878157-4877867 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT155071 NZ_CP068618:c5277423-5277181 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: S1P416