Detailed information of TA system TA155065

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-ohsC/SymE(toxin)
• Location: 4250779..4251191
• Replicon: chromosome
• Accession: NZ_CP051725
• Organism: Escherichia coli strain SCU-112

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: HHJ29_RS20290
• Protein ID: WP_000132630.1
• Coordinates: 4250850..4251191 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	ohsC
Locus tag	-
Coordinates	4250779..4250855 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ29_RS20280	4247391..4248860	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
HHJ29_RS20285	4248860..4250629	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
-	4250779..4250855	-	77	NuclAT_6	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_6	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_6	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_6	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_7	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_7	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_7	-	Antitoxin
-	4250779..4250855	-	77	NuclAT_7	-	Antitoxin
HHJ29_RS20290	4250850..4251191	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
HHJ29_RS20295	4251238..4253331	-	2094	WP_000648234.1	DUF262 domain-containing protein	-
HHJ29_RS20300	4253429..4253509	-	81	WP_020233658.1	hypothetical protein	-
HHJ29_RS20305	4253737..4254657	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
HHJ29_RS20310	4254842..4256122	+	1281	WP_001304526.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T155065 WP_000132630.1 NZ_CP051725:4250850-4251191 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T155065 NZ_CP068618:1910529-1910631 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 77 bp

>AT155065 NZ_CP051725:c4250855-4250779 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin